JACKEDFORUMS

Melanotan I

Afamelanotide · MT-1 · MT-I · Afamelanotide · Scenesse · [Nle4, D-Phe7]-alpha-MSH · NDP-MSH

Tanning-focused melanocortin agonist — the pigment of MT-II with far less nausea or erections.

Typical dose

500 mcg

Daily during the 10-14 day loading phase, then 1-2x weekly for maintenance

Range

250–1000

mcg

Half-life

1 h

see Dosing

Evidence

Human trials

Subcutaneous

What it is

Melanotan I is a linear 13-amino-acid analogue of alpha-MSH, identical to the native hormone apart from a norleucine at position 4 and a D-phenylalanine at position 7, which is what gives it potency and resistance to enzymatic breakdown. As afamelanotide it is a licensed medicine — Scenesse, a 16 mg controlled-release implant approved in the EU and US for erythropoietic protoporphyria.

The practical difference from Melanotan II is not receptor selectivity — afamelanotide is a superpotent but essentially non-selective agonist across MC1R, MC3R, MC4R and MC5R — it is pharmacokinetics. MT-I is a linear peptide that clears quickly and reaches the hypothalamus poorly, where MT-II's cyclic lactam is far more stable and centrally active, so users report little or no nausea, no spontaneous erections and no appetite suppression. The trade-off is that it takes more milligrams for the same tan and does nothing at all for libido.

The skin cancer caution carries over unchanged. Stimulating melanocytes with an unlicensed injectable while deliberately increasing UV exposure darkens existing moles and has been associated in case reports with new and changing naevi. Baseline mole mapping and annual dermatological review are the minimum sensible precautions.

How it works

MT-I is a potent agonist at MC1R on melanocytes. Binding raises cyclic AMP, upregulates tyrosinase and shifts melanin synthesis toward eumelanin, so the same UV dose produces a darker, faster tan and less erythema. In erythropoietic protoporphyria that increased eumelanin is what buys patients pain-free time in sunlight.

Its receptor profile is not actually MC1R-selective — NDP-MSH is a superpotent pan-agonist with high affinity at MC1R, MC3R, MC4R and MC5R alike. What limits the hypothalamic effects — nausea, flushing, erections, appetite loss — at tanning doses is pharmacokinetic rather than pharmacodynamic: a linear peptide that is cleared fast and penetrates the CNS poorly, where MT-II's cyclic lactam is metabolically stable and centrally active.

Dosing

Typical single dose500 mcg
Reported range250–1000 mcg
FrequencyDaily during the 10-14 day loading phase, then 1-2x weekly for maintenance
Injections per week7
Half-life~0.8-1.7 hours terminal after subcutaneous injection of the free peptide; the licensed Scenesse implant has an apparent half-life around 15 hours
TimingAny time of day — without the nausea and erections of MT-II there is no reason to push it to bedtime, though evening dosing pairs conveniently with next-morning UV exposure.
Typical run length10-14 day load, then maintenance dosing as needed
RoutesSubcutaneous
Molecular weight1647 Da
SequenceAc-Ser-Tyr-Ser-Nle-Glu-His-D-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH2

MT-I is weaker per milligram than MT-II, so the same 250 mcg that would floor you on MT-II barely registers here; expect to use roughly twice the milligrams for a comparable tan.

Reconstitution calculator

Pre-loaded with Melanotan I’s vial size, water volume and typical dose. Change anything.

Typical: 500 mcg · range 250–1000

05101520253020 units0.3 mL insulin · U-100 · half-unit marks

Draw to

20 u

0.2 mL · 500 mcg

Concentration

2.5 mg/mL

25 mcg per unit

Doses per vial

10

Vial lasts

10 days

Draw to 20 units on the barrel.

Once mixed
Refrigerated at 2-8°C, use within about 4 weeks. Keep it out of light; do not freeze.

Full calculator →
Suggested water2 mL per 5 mg vial
Common vial sizes5 mg, 10 mg

2 mL into a 5 mg vial gives 2,500 mcg/mL, so 500 mcg is 20 units on a U-100 pin; the same 2 mL into a 10 mg vial gives 5,000 mcg/mL and 500 mcg becomes 10 units. If you prefer easier measurement at 250 mcg, use 5 mL in the 10 mg vial for 2,000 mcg/mL. Direct the water at the glass wall and swirl until clear rather than shaking.

Storage

Before mixing

Refrigerated at 2-8°C it is stable for years; sealed and dry it survives several weeks at room temperature.

After mixing

Refrigerated at 2-8°C, use within about 4 weeks. Keep it out of light; do not freeze.

Reported benefits

  • Deep tan with substantially less UV exposure than tanning unaided
  • Essentially none of the nausea, flushing or erections that come with MT-II
  • No appetite suppression, so it does not interfere with a bulk
  • Backed by real human trial data and a licensed medicine (Scenesse)
  • Reduces phototoxic pain and burning in erythropoietic protoporphyria
  • Tan persists for weeks after the last dose

Side effects

  • Darkening of existing moles, freckles and old scars
  • New or changing melanocytic naevi with continued use
  • Mild nausea or flushing in a minority, usually only on the first few doses
  • Injection site reactions and transient fatigue
  • Uneven pigmentation, particularly on the face and hands
  • More injections needed than MT-II to hold the tan — a potency and stability difference in practice, not a half-life one (both peptides clear in roughly an hour)

Do not use if

  • History of melanoma, or atypical or dysplastic naevi
  • Numerous or unmapped moles you have never had assessed
  • Significant hepatic impairment, which is a labelled contraindication for the approved implant
  • Pregnancy or breastfeeding
  • Immunosuppression, including transplant recipients

Evidence level — Human trials

Controlled human clinical data exists.

Approved as Scenesse (afamelanotide) implant for erythropoietic protoporphyria in the US and EU; the injectable vialled form sold to the peptide market is not a licensed product.

Melanotan I FAQ

Why choose MT-I over MT-II?+

If you only want the tan, MT-I gives it without the nausea, unwanted erections and appetite loss that come with MT-II's central MC4R activity. The cost is more injections, more milligrams for the same result, and no libido effect at all.

Do I need UV exposure?+

Yes. MT-I primes the melanocytes but UV is what actually triggers pigment output, so dosing without any sun or bed exposure produces very little. Short, controlled exposures during the loading phase are the standard approach — you can still burn on day one.

How does the vialled peptide compare with the Scenesse implant?+

Same molecule, completely different delivery. The implant is given every two months, but it does not trickle out over that whole window — most of the 16 mg is released within the first 48 hours, over 90% by day 5, plasma levels are below the limit of quantitation by about day 10, and the polymer itself is absorbed by the body over 50-60 days. That release phase is what gives the apparent half-life near 15 hours, whereas injected free peptide clears in a couple of hours and has to be redosed daily during loading. Blood levels from injections are far spikier than the licensed product's.

Will it help with libido like PT-141?+

No. Not because it misses MC4R — afamelanotide binds the central receptors about as well as MT-II does — but because as a linear peptide it is cleared quickly and reaches the hypothalamus poorly, so in practice it is a tanning compound only. If libido is the goal, PT-141 is the compound with the data behind it.

Is the mole risk lower than with MT-II?+

There is no evidence it is. Both work by stimulating melanocytes and both have case reports of darkening moles and eruptive naevi, so the same precautions apply: photograph and map your moles before starting, and have a dermatologist review anything that changes.

References

  1. 1.Afamelanotide for Erythropoietic ProtoporphyriaThe New England Journal of Medicine (2015) PMID 26132941
  2. 2.Skin pigmentation and pharmacokinetics of melanotan-I in humansBiopharmaceutics & Drug Disposition (1997) PMID 9113347
  3. 3.Afamelanotide: A Review in Erythropoietic ProtoporphyriaAmerican Journal of Clinical Dermatology (2016) PMID 26979527
  4. 4.Eruptive melanocytic naevi following melanotan injectionThe British Journal of Dermatology (2009) PMID 19575725

More sexual health & melanocortin