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Selank

Threonyl-lysyl-prolyl-arginyl-prolyl-glycyl-proline · TP-7 · Selank 0.15% · Tuftsin-PGP

Russian anxiolytic heptapeptide used for anxiety without benzo sedation or dependence.

Typical dose

300 mcg

2x daily (Russian trials used 3x daily)

Range

150–900

mcg

Half-life

see Dosing

Evidence

Early human

Intranasal · Subcutaneous

What it is

Selank is a synthetic heptapeptide built at the Institute of Molecular Genetics in Moscow by taking tuftsin (the immune-active tetrapeptide Thr-Lys-Pro-Arg) and bolting a Pro-Gly-Pro tail onto the C-terminus. That tail is the whole trick: it blocks the aminopeptidases that would otherwise chew the parent peptide up in seconds, and it improves passage into the brain.

It has been registered in Russia since 2009 as a 0.15% intranasal solution for generalised anxiety disorder and neurasthenia. Russian trials reported anxiolytic efficacy in the same range as medazepam and phenazepam without sedation, tolerance or a withdrawal syndrome. Those trials are small, mostly from one research group, and have never been replicated in a Western regulatory-grade study.

In practice people use it as a take-the-edge-off compound rather than a stimulant: reduced anxiety and rumination, slightly better focus under stress, no cognitive dulling. It is not a benzodiazepine substitute for anyone physically dependent, and it will not touch a panic disorder on its own.

Effects tend to be subtle and stack over a two-week course rather than hitting hard on day one. People expecting an acute hit usually conclude it does nothing.

How it works

Selank's clearest documented action is inhibition of enkephalin-degrading enzymes, which raises endogenous enkephalin tone — a plausible route to anxiolysis that does not involve direct receptor agonism. It also shifts expression of GABA-A receptor subunits and GABA-transporter genes in rodent brain, which is why its effects overlap with benzodiazepines without the receptor binding that drives tolerance and dependence.

Secondary effects include modulation of monoamine turnover, changes in BDNF expression, and immune-modulating activity inherited from the tuftsin fragment (interleukin-6 and interferon-related). The exact contribution of each pathway to the subjective effect has not been pinned down.

Dosing

Typical single dose300 mcg
Reported range150–900 mcg
Frequency2x daily (Russian trials used 3x daily)
Injections per week14
Half-lifeMinutes, not hours — in-vitro rodent plasma estimates cluster around 2-5 minutes. Downstream gene-expression and enkephalin effects persist for hours to days after the peptide itself is gone. No verified human PK exists.
TimingSplit between nostrils, roughly half the volume per side. Anxiolytic effect is usually noticed 20-40 minutes in. No food timing requirement.
Typical run length2-4 weeks
RoutesIntranasal, Subcutaneous
Molecular weight752 Da
SequenceTKPRPGP

The registered Russian 0.15% product is dosed at 300-900 mcg per administration (4-12 drops), three times a day, giving a daily total of 900-2,700 mcg over a 10-14 day course. The 300 mcg x 3 = 900 mcg/day figure usually quoted is the bottom of that range, not the ceiling. Injection offers no advantage over intranasal here, and the peptide is cleared by plasma and mucosal peptidases within minutes either way.

Reconstitution calculator

Pre-loaded with Selank’s vial size, water volume and typical dose. Change anything.

Typical: 300 mcg · range 150–900

05101520253012 units0.3 mL insulin · U-100 · half-unit marks

Draw to

12 u

0.12 mL · 300 mcg

Concentration

2.5 mg/mL

25 mcg per unit

Doses per vial

16.7

Vial lasts

8 days

Draw to 12 units on the barrel.

Once mixed
Refrigerated at 2-8 °C, used within 3-4 weeks. Nasal spray bottles kept at room temperature degrade faster — keep the working bottle cold between uses.

Full calculator →
Suggested water2 mL per 5 mg vial
Common vial sizes5 mg, 10 mg

2 mL of bacteriostatic water into a 5 mg vial gives 2,500 mcg/mL, so a 300 mcg dose is 0.12 mL. Aim the water down the vial wall and swirl — do not shake. Many people transfer the reconstituted solution into a metered nasal spray bottle; check the bottle's per-actuation volume before assuming a dose.

Storage

Before mixing

Freezer at -20 °C for long-term storage (24+ months); stable in a fridge for months and survives ambient shipping.

After mixing

Refrigerated at 2-8 °C, used within 3-4 weeks. Nasal spray bottles kept at room temperature degrade faster — keep the working bottle cold between uses.

Reported benefits

  • Reduces anxiety and rumination without sedation or cognitive blunting
  • No tolerance, dependence or withdrawal reported across Russian trial data
  • Modestly improves attention and working memory under stress rather than at baseline
  • Raises endogenous enkephalin tone via enkephalinase inhibition
  • Anecdotally useful as support during a benzodiazepine or alcohol taper
  • Stacks cleanly with stimulating nootropics to blunt their anxiety edge

Side effects

  • Nasal burning, irritation and runny nose — the most common complaint
  • Bitter taste draining down the back of the throat
  • Mild headache in the first few days
  • Transient fatigue or emotional flatness at higher daily totals
  • Occasional low mood on stopping after long continuous use
  • No long-term human safety data beyond 14-30 day Russian courses

Do not use if

  • Pregnancy and breastfeeding — no data
  • Physical benzodiazepine dependence — Selank does not prevent withdrawal and should not replace a supervised taper
  • Caution alongside opioids, given the enkephalinase inhibition and unstudied interaction
  • Active autoimmune disease, given the tuftsin-derived immune modulation

Evidence level — Early human

Small or early-phase human studies only.

Registered as a prescription intranasal drug in Russia; not FDA-approved and sold elsewhere as a research chemical.

Commonly run with

Selank FAQ

Is Selank actually as good as a benzo?+

No. Russian trials reported comparable scores on anxiety rating scales versus medazepam and phenazepam, but those were small and largely unblinded. Practically it takes the edge off background anxiety over a couple of weeks; it will not abort a panic attack the way a benzodiazepine does.

Intranasal or injected?+

Intranasal. Rodent work puts intranasal bioavailability very high and it is the route used in every registered Russian protocol. Subcutaneous injection works but adds needles for no measurable benefit, since the peptide is degraded within minutes either way.

What is N-Acetyl Selank Amidate?+

It is a chemically modified analogue with an acetylated N-terminus and amidated C-terminus, sold as being more stable and more potent per microgram. It is a different molecule with essentially no published data of its own, so dose figures for Selank do not transfer to it directly.

How long before it does anything?+

Most people notice the anxiolytic effect within an hour of the first dose but describe it as subtle. The more reliable effect builds over 5-14 days of consistent dosing, which matches the gene-expression timescale seen in animal work.

Do I need to cycle it?+

Trial protocols ran 14 days and stopped. There is no tolerance mechanism that forces a break, but there is also no safety data past about a month, so 2-4 week courses with a break is the conservative default.

References

  1. 1.Peptide-based Anxiolytics: The Molecular Aspects of Heptapeptide Selank Biological ActivityProtein and Peptide Letters (2018) PMID 30255741
  2. 2.Selank Administration Affects the Expression of Some Genes Involved in GABAergic NeurotransmissionFrontiers in Pharmacology (2016) PMID 26924987
  3. 3.Peptide Selank Enhances the Effect of Diazepam in Reducing Anxiety in Unpredictable Chronic Mild Stress Conditions in RatsBehavioural Neurology (2017) PMID 28280289
  4. 4.Functional Connectomic Approach to Studying Selank and Semax EffectsDoklady Biological Sciences (2020) PMID 32342318

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