Hello guys !
I will post in this thread HPLC reports from our laboratory for each batch and HPLC from external lab’s time to time.
But first of all few words about HPLC and about GH:
Here is short explanation of HPLC:
Purity: RP-HPLC (EP 8.0) should be> 94% → the content of similar proteins with less biological activity than GH, in acc.with Pharmacopoeia concentration of similar proteins should be less than 6%.
Dimers and aggregates: SEC-HPLC (EP 8.0) should be> 96% → High molecular weight admixtures of somatotropin and products of its physical degradation.
It characterizes the immunogenicity of the drug and ability to induce an immune response. The more concentration - the stronger immune response neutralizing growth hormone, in acc.with Pharmacopoeia concentration of dims/aggs should be less than 4%.
Concentration of HGH → I think not need to explain
One more important characteristic is a Endotoxins (didn’t show in this report) - molecular components of membranes of microorganisms, which have a toxic effect on the mammalian organism. Endotoxins can provoke chronic hyperactivation of the immune system and lead to critical consequences for the whole organism. In acc.with Pharmacopoeia concentration should be less than 5 UI/mg.
“3D” model of HGH (or peptide map) → showed actual model of HGH in three-dimensional space (rotations, angles, geometry). Must coincide with the natural form (as accurately as possible). In the opposite case → GH in your blood after injection will be raised but not worked (your blood test for GH serum will be OK, but in fact this GH for nothing)
May be I missed something. But 5 main things you should know as minimum
So, here is HPLC made in our lab. for current batch:
Here is HPLC made by external lab. for current batch:
And one more:
“peptide knife” test. HGH is cut by peptide knife and each peak on this graphic it’s a molecular body in HGH structure. And if peaks of some manufactued HGH absolutely the same with natural human GH we can conclude that it’s absolutely identical in terms of activity/effects … etc
On the graphics: left side you can see structure of hormone which is stored in Belgium and is a benchmark for manufacturers, right side our Neofin
I will post in this thread HPLC reports from our laboratory for each batch and HPLC from external lab’s time to time.
But first of all few words about HPLC and about GH:
Here is short explanation of HPLC:
Purity: RP-HPLC (EP 8.0) should be> 94% → the content of similar proteins with less biological activity than GH, in acc.with Pharmacopoeia concentration of similar proteins should be less than 6%.
Dimers and aggregates: SEC-HPLC (EP 8.0) should be> 96% → High molecular weight admixtures of somatotropin and products of its physical degradation.
It characterizes the immunogenicity of the drug and ability to induce an immune response. The more concentration - the stronger immune response neutralizing growth hormone, in acc.with Pharmacopoeia concentration of dims/aggs should be less than 4%.
Concentration of HGH → I think not need to explain
One more important characteristic is a Endotoxins (didn’t show in this report) - molecular components of membranes of microorganisms, which have a toxic effect on the mammalian organism. Endotoxins can provoke chronic hyperactivation of the immune system and lead to critical consequences for the whole organism. In acc.with Pharmacopoeia concentration should be less than 5 UI/mg.
“3D” model of HGH (or peptide map) → showed actual model of HGH in three-dimensional space (rotations, angles, geometry). Must coincide with the natural form (as accurately as possible). In the opposite case → GH in your blood after injection will be raised but not worked
(your blood test for GH serum will be OK, but in fact this GH for nothing)May be I missed something. But 5 main things you should know as minimum
So, here is HPLC made in our lab. for current batch:
Here is HPLC made by external lab. for current batch:
And one more:
“peptide knife” test. HGH is cut by peptide knife and each peak on this graphic it’s a molecular body in HGH structure. And if peaks of some manufactued HGH absolutely the same with natural human GH we can conclude that it’s absolutely identical in terms of activity/effects … etc
On the graphics: left side you can see structure of hormone which is stored in Belgium and is a benchmark for manufacturers, right side our Neofin
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